Back in the 1960s, scholars in the field of biochemistry were hard pressed to find the types of sophisticated equipment they needed for their pioneering research. Atto Corporation was established as a specialist manufacturer in 1964 to meet their demands. The company founders had every confidence that the field of biochemistry was destined to grow. In the four decades since, Atto has invested substantial resources and money in research and development under the guidance of leading biochemists.
Since our establishment, we have continued to develop, manufacture, and sell biological macromolecules and analytical products such as chromatographs and electrophoresis apparatus. Over the last decade we have also developed and marketed a luminescence device for the research of gene expression.
Our name Atto comes from “atto,” an incredibly small unit equal to 1 x 10-18, or one-quintillionth. Modern scientists and technologists now hope to delve deeper into exploratory research on the “atto” scale. This is a field Atto is poised to enter.
In recent years, the Japanese government has begun to promote joint research between universities and enterprises. However, Atto has been engaged in collaboration with universities, and has already achieved several fruitful results to date. One of the results of collaboration won an award from the Ministry of Education, Culture, Sports, Science and Technology in 2001. 日本ATTO株式会社产品目录：
Wide partition walls between wells improve sealing
Developed in conjunction with the MRC Laboratory of Molecular Biology in Cambridge, United Kingdom
UV compatible (UVP)
Description MRC Maxi 48-Well Crystallization Plate for Automated Optimization
The MRC Maxi optimization plate is a breakthrough for macromolecular crystallization presented in a 48 well format. Offering easy to automate crystallization optimization with large sitting-drops, the new MRC Maxi Crystallization plate is the perfect solution. Manufactured by Swissci AG, the plate offers an SBS format while providing 48 wells. MRC Maxi is intended for large drops and is compatible both with standard robotic systems as well as manual pipetting.
The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) in collaboration with Jan Löwe and Fabrice Gorrec. It is a result of many years of experience in successful robotic high-throughput crystallization and complements the original MRC crystallization plate, which is intended for smaller drop volumes and higher throughput during screening.
Drop volumes of up to 10 µl are possible. The 9 mm standard distance between wells is preserved, enabling the use of multi-channel manual pipettes and robotic liquid handlers, making MRC Maxi one of the most automation-friendly optimization plates on the market.
MRC Maxi is covered by global intellectual property and design registration as are the Swissci AG MRC 2 lens 96 well plates. Several breakthrough features of the original MRC plate have been maintained. Wells are labeled individually. Drops are raised for easy access during crystal retrieval. MRC Maxi uses the same proprietary polymer specially selected for the purpose of UV light visualization and the material used keeps through-plastic evaporation to a minimum. Well shapes are spherical but shallow.
The MRC Maxi Crystallization plate offers unique properties that make it the ideal choice for microliter-sized optimization experiments and is made from UV compatible UVP.
The advantages of the MRC Maxi Crystallization plate – in brief
Easy Crystal Retrieval
Raised wide wells make the crystal mounting especially easy.
The wells are wide conical.
Each well has a micro lens for perfect illumination.
Micro numbering readable under the microscope for each well.
The optically superior polymer (UVP) is UV transmissible.
Wide partition walls between the wells give plenty of area for good sealing with tape.
Very Rigid, Automation-Friendly Plate Design
The UVP polymer reduces through-plastic evaporation to a minimum.
The plates are designed to the SBS standard and are compatible with all common holders.
9 mm distance from well-to-well within columns, 18 mm distance within rows.
Unique Polymer (UVP)
Ultra-low sample binding.
No static charging.
Volumes validated for MRC Maxi are up to 10 µl of sample drop and 200 µl of the crystallization reagent.
Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
Salt versus pH matrix crystallization screen
Samples pH 4 to 9
22 unique salts versus salt concentration and pH
Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods
Tube or Deep Well block format
Preformulated, ready to screen
Developed at Hampton Research
All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals
Individual reagents available through the Hampton Research Custom Shop
Description SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C
Sitting drop crystallization
96 well sitting drop plate
Round and conical flat bottom drop well shapes
1, 2 or 4 microliter drop wells
1, 3 or 5 drop wells per reservoir
Hydrophilicity treated or untreated
PZero or COC plate material–UV imaging compatible
The second generation of Corning® 96 Well, sitting drop format plates are built to SBS specifications, making them well suited for high throughput crystallization and are fully compatible with robotic equipment. The plates are available in several different configurations with varying drop well shapes, plate materials, and number of drop wells per reagent well. The basic plate design is one reagent well flanked by one or three drop wells, with SBS standard spacing between the centers of adjacent well clusters. One may choose a plate with small (1 µl), medium (2 µl), or large (4 µl) drop well volumes. The choice of round or conical flat well shapes are available. The PZero polymer provides for zero background polarization and is non-birefringent. PZero plates are not treated. The COC polymer offers high chemical resistance. Both types of plastic feature improved transparency. The reservoir numbers are embossed on each individual well for easy identification. Drop well locations conform to SBS standards for robotic handling. The low-volume reagent well saves on reagent costs. The plates can be sealed using Crystal Clear Sealing Film (HR3-609), 3 inch wide Crystal Clear Sealing Tape (HR4-506) or ClearSeal Film™ (HR4-521).
Corning has discontinued 3557 – special pricing and limited inventory available for this item only.
4µl round drop well, 1 drop well, COC, untreated – 10 plate case
4 µl round drop well, 1 drop well, COC, untreated – 40 plate case
4 µl conical flat drop well, 1 drop well, COC, treated – 10 plate case
4µl conical flat drop well, 1 drop well, COC, treated – 40 plate case
2 µl round drop well, 3 drop well, COC, untreated – 10 plate case
2µl round drop well, 3 drop well, COC, untreated – 40 plate case
2 µl conical flat drop well, 3 drop well, COC, untreated – 10 plate case
2 µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
1µll conical flat drop well, 3 drop well, COC, untreated – 10 plate case
1µl conical flat drop well, 3 drop well, COC, untreated – 40 plate case
1 µl conical flat drop well, 5 drop well, PZero – 10 plate case
1 µl conical flat dro p well, 5 drop well, PZero – 40 plate case